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Top10 electrocompetent E. coli cells were transformed with the P32 cassette assembly and plated on LB + CAM plates. After one day of incubation at 37°C, there was growth of purple-blue …
Customer ServiceIn the resulting mutants, the target gene is replaced by a lox66-P32-cat-lox71 cassette, where lox66 and lox71 are mutant variants of loxP and P32-cat is a chloramphenicol …
Customer ServiceTo face this problem, we removed the resistance cassette using natural transformation by designing only homology arms, creating a recombination removing the P32 …
Customer ServiceTeflon / PFA Cassette PP / PEI / PC Cassette Metal / PEEK Cassette Shipping Box / Single Tray / Storage Box 150mm (6) 200mm (8) …
Customer ServicePlasmid pJet1.2 cat cassette from Dr. Stephan Gruber''s lab contains the insert Chloramphenicol resistance cassette for Bacillus subtilis selection and is published in Methods Mol Biol. …
Customer ServiceThe P32- cat cassette (1.3 kb) was amplified with primers Uplox66 and DNlox71 using plasmid pNZ5319 as a template (30). The UP and DN regions (1.2 kb) were PCR amplified using the …
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